Manfred Heinlein

Work Address: Institute of Botany University of Basel Hebelstrasse 1, 4056 Basel Switzerland Phone +41 (61) 267 23 24 Fax +41 (61) 267 23 30 Email: manfred.heinleinunibas.ch http://plantbiology.unibas.ch/rnasignaling/rnasignaling.htm

Academic education

1981-1986	Undergraduate studies in Biology, University of Cologne, Germany
1985-1986	Diploma thesis, University of Cologne
1986	Diploma in Biology, University of Cologne
1987-1992	Ph.D. thesis, Institute of Genetics, University of Cologne
2001	Habilitation (Venia Docendi) in Molecular Biology, University of Basel, Switzerland

Research experience and affiliations

1983-1985

Laboratory work in between terms:
Department of Prof. Dr. Jozef Schell
Max-Planck-Institute for Plant Breeding Research, Cologne, Germany.

1985-1986: Diploma thesis:

"Tissue-specific expression of the Sucrose Synthase isoenzymes of Zea mays L. and the demonstration of Sh- sectors in the endosperm of mutants carrying the unstable sh-m5933 allele."
Laboratory of Prof. Dr. Peter Starlinger, Institute of Genetics, University of Cologne

1987-1992: Ph.D. thesis:

"Genetic studies on the regulation of Ac and Ds transposition in Zea mays L."
Laboratory of Prof. Dr. Peter Starlinger, Institute of Genetics, University of Cologne, with visits to:
Laboratories of Prof. Dr. Mario Motto and Prof. Dr. Francesco Salamini, Istituto Sperimentale per la Cerealicoltura, Bergamo, Italy
Dr. Barbara McClintock, Cold Spring Harbor, N.J. USA

1992-1994: Assistant Scientist

Laboratory of Prof. Dr. Peter Starlinger, Institute of Genetics, University of Cologne
- Structure-function analysis of Ac transposase
- in situ detection of Ac transposase
- methylation of transposase binding sites in Ac

1994-1997: Post-doctoral Research

Laboratory of Roger N. Beachy, Ph.D. The Scripps Research Institute (TSRI), La Jolla, CA, USA.
- Studies on Tobacco mosaic virus movement protein
- First functional GFP fusion protein in plant biology
- Involvement of the plant cytoskeleton in virus movement

1997-2003: Group leader

Friedrich Miescher Institute for Biomedical Research (FMI), Basel, Switzerland
- Intra- and intercellular transport of RNA in plants
- Macromolecular transport and long-distance signaling
- RNA silencing, virus-induced silencing (VIGS)
- Plant-virus interactions, cytoskeleton, plasmodesmata, host factors
- Arabidopsis gene arrays, proteomics
- Mechanism of induced or engineered resistance of plants to viruses

Since 2003: Assistant Professor

Institute of Botany, University of Basel, Switzerland

Since 2004: Directeur de Recherche

Institut de Biologie Moleculaire des Plantes (IBMP, CNRS UPR2357), Strasbourg, France

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Publications:

 

2010

• Niehl A., and Heinlein M. (2010): Cellular pathways for viral transport through plasmodesmata. Protoplasma , in press.
• Amari K., Boutant E., Hofmann C., Schmitt-Keichinger C., Fernandez-Calvino L., Didier P., Lerich A., Mutterer J., Thomas C., Heinlein M., Mély Y., Maule A.J., and Ritzenthaler C. (2010): A family of plasmodesmal proteins with receptor-like properties for plant viral movement proteins. PLoS Pathog. 6, e1001119.
• Ouko M.O., Sambade A., Brandner K., Niehl A., Pena E., Ahad A., Heinlein M., and Nick P. (2010): Tobacco mutants with reduced microtubule dynamics are less susceptible to TMV. Plant J. 62, 829-839.
• Boutant E., Didier P., Niehl A., Mély Y., Ritzenthaler C., and Heinlein M. (2010): Fluorescent protein recruitment assay for demonstration and analysis of in vivo protein interactions in plant cells and its application to Tobacco mosaic virus movement protein. Plant J. 62, 171-177.
• Bolze F., Niehl A., Heinlein M., Mjdasiri N., Rehspringer J.L., Schaeffer N., Didier P., Arnitz Y., Mély Y., Gug S., Specht A., Goeldner M., and Nicoud J.F. (2010): Two-photon excitation in the life sciences: From observation to action. Nonlinear Optics, Quantum Optics 40, 253-265.

 

2009

• Boutant E., Fitterer C., Ritzenthaler C., and Heinlein M. (2009): Interaction of the Tobacco mosaic virus movement protein with microtubules during the cell cycle in tobacco BY-2 cells. Plant Physiol. 237, 3-12.
• Heinlein M. (2009): Plant virus movement and the impact of RNA silencing. Enzyclopedia of Life Sciences.
• Hofmann C., Niehl A., Sambade A., Steinmetz A., and Heinlein M. (2009): Inhibition of Tobacco mosaic virus movement by expression of an actin-binding protein. Plant Physiol. 149, 1810-1823.
• Niehl A., and Heinlein M. (2009): Impact of RNA virus infection on plant cell function and evolution. Ann. NY Acad. Sci. 1178, 120-128.
• Sambade A., and Heinlein M. (2009): Approaching the cellular mechanism that supports the intercellular spread of Tobacco mosaic virus. Plant Signaling and Behavior 4, 35-38.

 

2008

• Sambade A., Brandner K., Hofmann C., Seemanpillai M., Mutterer J., and Heinlein M. (2008): Transport of TMV movement protein particles associated with the targeting of RNA to plasmodesmata. Traffic 9, 2073-2088.
• Brandner K., Sambade A., Boutant E., Didier P., Mély Y., Ritzenthaler C., and Heinlein M. (2008): Tobacco mosaic virus interacts with green fluorescent protein-tagged microtubule end-binding protein 1. Plant Physiol. 147, 611-623.
• Vogler H., Kwon M.-O., Dang, V.,  Sambade, A.,  Fasler, M.,  Ashby J., and Heinlein M. (2008): Tobacco mosaic virus movement protein enhances the spread of RNA silencing. PLoS Pathog. 4, e1000038.  
• Heinlein M. (2008): Microtubules and Viral Movement. In: Plant Microtubules: Development and Flexibility (2nd edition) (ed. P.Nick), Plant Cell Monographs, Springer, Berlin-Heidelberg, pp141-173.

 

2007

• Vogler H., Abergenov R., Shivaprasad R.V., Dang V., Fasler M., Kwon M.-O., Zhanybekova S., Hohn T., and Heinlein M. (2007): Modification of small sRNAs associated with suppression of RNA silencing by tobamovirus replicase protein. J. Virol. 81, 10379-10388. 
• Waigmann E., Curin M., and Heinlein M. (2007): Tobacco mosaic virus – a model for macromolecular cell-to-cell spread. In: Viral Transport in Plants (eds. E. Waigmann and M. Heinlein), pp 29-62, Plant Cell Monographs Vol. 7, Springer Berlin-Heidelberg.
• Boyko V., Hu Q., Seemanpillai M., Ashby J., and Heinlein M. (2007): Validation of microtubule-associated Tobacco mosaic virus RNA movement and involvement of microtubule-aligned particle trafficking. Plant J. 51, 589-603.
• Hofmann C., Sambade A., and Heinlein M. (2007): Plasmodesmata and intercellular transport of viral RNA. Biochem. Soc. Transact., 35, 142-145.

 

2006

• Ashby J., Boutant E., Seemanpillai M., Groner A., Sambade A., Ritzenthaler C., and Heinlein M (2006): Tobacco mosiac virus movement protein functions as a structural microtubule-associated protein. J. Virol., 80, 8329-8344.
• Seemanpillai M., Elamawi R., Ritzenthaler C. and Heinlein M. (2006): Challenging the role of microtubules in Tobacco mosaic virus movement by drug treatments is disputable. J. Virol., 80, 6712-1715.
• Ferralli J., Ashby J., Fasler M., Boyko, V. and Heinlein M. (2006): Disruption of microtubule organization and centrosome function by expression of Tobacco mosaic virus movement protein. J. Virol., 80, 5807-5821.
• Heinlein M. (2006): TMV movement protein targets cell-cell channels in plants and prokaryotes: possible roles  of tubulin- and FtsZ-based cytoskeletons. In: Cell-Cell-Channels (eds. F. Baluska, D. Volkmann, P.W. Barlow), Landes Bioscience, pp. 176-182.

 

2005

• Heinlein M. (2005): Systemic RNA silencing. In: Plasmodesmata. Ann. Plant Rev. 18 (ed. K. Oparka), Blackwell Publishing (Oxford), pp. 212-241.

 

2004

• Heinlein M. and Epel B.L. (2004): Macromolecular transport and signaling through plasmodesmata. Int. Rev. Cytol., 235, 93-164.

 

2003

• Kovalchuk I., Kovalchuk O., Kalck V., Boyko V., Filkowski, J., Heinlein M., and Hohn B. (2003) Pathogen-induced systemic signal triggers genome instability. Nature 423, 760-762.

 

2002

• Heinlein M. (2002) Plasmodesmata: dynamic regulation and role in macromolecular trafficking. Curr. Opin. Plant Biol. 5, 543-552.
• Heinlein M. (2002): The illustrious life of a tobacco pathogen. Book Review. Cell 109, 670-671.
• Boyko V., Ashby J.A., Suslova E., Ferralli J., Sterthaus O., Deom C.M., and Heinlein M. (2002) Intramolecular complementing mutations in Tobacco mosaic virus movement protein confirm a role of microtubule association in viral RNA transport. J. Virol. 76, 3974-3980.
• Chen W., Provart N., Chang H.S., Glazebrook J., Zou G., Whitham S., Katagiri F., Budworth P., Luan S., Lam S., Kreps J., Harper J., Mauch F., Dangl J., Heinlein M., Hohn T., Wang X., and Zhu T. (2002) Transcription profile matrix of Arabidopsis transcription factors reveals their functions in response to environmental stresses. Plant Cell 14, 559-574.
• Heinlein M. (2002) The spread of Tobacco mosaic virus infection: insights into the cellular mechanism of RNA transport. Cell. Mol. Life Sci. 59, 58-82.

 

2001

• Bucher G.L., Tarina C., di Serio F., Heinlein M., Meins F., and Iglesias V.A. (2001): Local expression of enzymatically active class I b-1,3-glucanase enhances local spread of TMV infection in tobacco. Plant J. 28, 361-369.
• Kotlizky G., Katz A., van der Laak J., Boyko V., Lapidot M., Beachy R.N., Heinlein M., and Epel B.L. (2001): A dysfunctional  movement protein of Tobacco mosaic virus  interferes with targeting of wild type movement protein to microtubules. Mol. Plant Microbe Interact 14, 895-904.

 

2000

 

• Boyko V., van der Laak J., Ferralli J., Suslova A., Kwon M.-O., and Heinlein M. (2000): Cellular targets of functional and dysfunctional mutants of Tobacco mosaic virus movement protein fused to GFP. J.Virol. 74, 11339-11346.
• Boyko V., Ferralli J., Ashby J., Schellenbaum P., and Heinlein M. (2000): Function of microtubules in intercellular transport of plant virus RNA. Nature Cell Biol. 2, 826-832.
• Beachy R.N. and Heinlein M. (2000): Role of P30 in replication and spread of TMV. Traffic 1, 540-544.
• Boyko V., Ferralli, J., and Heinlein M. (2000): Cell-to-Cell movement of TMV RNA is temperature-dependent and corresponds to the association of movement protein with microtubules. Plant J. 22, 315-325.

 

1998

• Kahn T.W., Lapidot M., Heinlein M., Reichel C., Cooper B, Gafny R., and Beachy R.N. (1998): Domains of the TMV movement protein involved in subcellular localization. Plant J. 15, 15-25.
• Heinlein M., Padgett H.S., Gens J.S., Pickard B.G., Casper S.J., Epel B.L., and Beachy R.N. (1998): Changing patterns of localization of the Tobacco mosaic virus movement protein and replicase to endoplasmic reticulum and microtubules during infection. Plant Cell 10, 1107-1120.
• Heinlein M., Wood M.R., Thiel T. and Beachy R.N. (1998): Targeting and modification of prokaryotic cell-cell junctions by Tobacco mosaic virus cell-to-cell movement protein. Plant J. 14, 345-351. 

 

1997

• Beachy R. N., Padgett H.S., Kahn T., Bendahmane M., Fitchen J.H., Heinlein M., Watanabe Y., and Epel B.L. (1997): Cellular and molecular mechanisms of coat protein and movement protein mediated resistance against TMV. In: Biology of Plant-Microbe Interactions. Stacey M., Mullin B., Gresshoff P.M. (eds.), IS-MPMI, St. Paul, MN, USA.

 

1996

• Padgett H.S., Epel B.L., Kahn T.W., Heinlein M., Watanabe Y., and Beachy R.N. (1996): Distribution of tobamovirus movement protein in infected cells and implications for cell-to-cell spread of infection. Plant J. 10, 1079-1088.
• Heinlein M. (1996): Excision patterns of Activator (Ac)- and Dissociation (Ds)-elements in Zea mays L.: implications for the regulation of transposition. Genetics 144, 1851-1869.
• Epel B.L., Padgett H.S., Heinlein M., and Beachy R.N. (1996): Plant virus movement protein dynamics probed with a GFP-protein fusion. Gene 173, 75-79.
• Wang L., Heinlein M., and Kunze R. (1996): Methylation pattern of Activator (Ac) transposase binding sites in maize endosperm. Plant Cell 8, 747-758.

 

1995

• Heinlein M., Epel B.L., Padgett H.S., and Beachy, R.N. (1995): Interaction of tobamovirus movement proteins with the plant cytoskeleton. Science 270, 1983-1985.
• Boehm U., Heinlein M., Behrens U., and Kunze R. (1995): One of three nuclear localization signals of maize Activator  (Ac) transposase overlaps the DNA-binding domain. Plant J. 7, 441-451.
• Heinlein M. (1995):Variegation patterns caused by excision of the maize transposable element Dissociation (Ds) are autonomously regulated by allele-specific Activator  (Ac ) elements and are not due to trans-acting modifier genes. Mol. Gen. Genet 246, 1-9.

 

1994

• Heinlein M., Brattig T., and Kunze R. (1994): In vivo aggregation of maize Activator  (Ac ) transposase in nuclei of maize endosperm and Petunia protoplasts. Plant J. 5, 705-714.

 

1991

• Heinlein M. and Starlinger P. (1991): Variegation patterns caused by transposable element Ac. Maydica 36, 309-316.

 

1989

• Starlinger P., Coupland G., Fusswinkel H., Heinlein M., Kunze R., Laufs J., Li M., Stochaj U., Baker B., Schell J., Both C., Oellig C., Doerfler W., and Schwartz D. (1989): Studies on transposable element Ac in  Zea mays. In: The molecular basis of plant development. UCLA Symp. on molecular and cellular biology, New Series Vol. 92 (R. Goldberg, ed.), Alan R. Liss Inc., NY, pp. 65-75.
• Heinlein M. and Starlinger P. (1989): Tissue- and cell-specific expression of the two sucrose synthase isoenzymes in developing maize kernels. Mol. Gen. Genet. 215, 441-446.

 

1988

• Hauser C., Fusswinkel H., Li J., Oellig C., Kunze R., Mueller-Neumann M., Heinlein M., Starlinger P., and Doerfler W. (1988): Overproduction of the protein encoded by the maize transposable element Ac in insect cells by a baculovirus vector. Mol. Gen. Genet. 214, 373-378.

 

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