We have a long-standing interest in the biology of fructan accumulation and metabolism in plants. Fructans are polymers of fructose ultimately derived from the fructosyl moiety of sucrose, and they are synthesized by a number of different fructosyl transferases. Recently, a number of cDNAs have been cloned representing both fructosyl transferases as well as fructan hydrolases from various plants. Sequence comparison has shown that all these enzymes are most likely derived from a primordial invertase, i.e. a hydrolase cleaving sucrose.
- Altenbach, D., E. Nüesch, A.D. Meyer, T. Boller, A. Wiemken. 2004: The large subunit determines catalytic specificity of barley sucrose:fructan 6-fructosyltransferase and fescue sucrose:sucrose 1-fructosyltransferase. FEBS Letters 567: 214-218
- Hochstrasser, U., Lüscher, M., De Virgilio, C., Boller, T. and Wiemken, A. (1998) Expression of a functional barley sucrose-fructan 6-fructosyltransferase in the methylotrophic yeast Pichia pastoris FEBS Lett. 440:356-360
- Lüscher, M., Erdin, C., Sprenger, N., Hochstrasser, U., Boller, T. and Wiemken, A. (1996) Inulin synthesis by a combination of purified fructosyltransferases from tubers of Helianthus tuberosus. FEBS Lett. 385:39-42
- Lüscher, M., U. Hochstrasser, T. Boller, A. Wiemken, 2000: Isolation of sucrose:sucrose 1-fructosyltransferase (1-SST) from barley (Hordeum vulgare). New Phytol. 145, 225- 232.
- Müller, J., U. Mohr, N. Sprenger, K. Bortlik, T. Boller, A. Wiemken, 1999: Pool sizes of fructans in roots and leaves of mycorrhizal and non-mycorrhizal barley. New Phytol. 142, 551-559.
- Müller, J., R.A. Aeschbacher, N. Sprenger, T. Boller, A. Wiemken, 2000: Disaccharide-mediated regulation of sucrose:fructan-6-fructosyltransferase, a key enzyme of fructan synthesis in barley leaves. Plant Physiol. 123: 265- 274.
- Nagaraj, V.J., R. Riedl, T. Boller, A. Wiemken, A.D. Meyer, 2001: Light and sugar regulation of the barley sucrose:fructan 6-fructosyltransferase promoter. J. Plant Physiol. 158, 1601- 1607.
- Nagaraj, V.J., V. Galati, M. Lüscher, T. Boller, A. Wiemken. 2005. Cloning and functional characterization of a cDNA encoding barley soluble acid invertase (HvINV1). Plant Science 168: 249-258
- Nagaraj V.J., D. Altenbach, V. Galati, M. Lüscher, A. D. Meyer, T. Boller, A. Wiemken. 2004. Distinct regulation of sucrose: sucrose-1-fructosyltransferase (1-SST) and sucrose: fructan-6-fructosyltransferase (6-SFT), the key enzymes of fructan synthesis in barley leaves: 1-SST as the pacemaker. New Phytologist 161: 735-748
- Roth, A., Lüscher, M., Sprenger, N., Boller, T. and Wiemken, A. (1996) Fructan and fructan-metabolizing enzymes in the growth zone of barley leaves. New Phytol. 136:73-79
- Schellenbaum, L., N. Sprenger, H. Schüepp, A. Wiemken, T. Boller, 1999: Effects of drought, transgenic expression of a fructan synthesizing enzyme and of mycorrhizal symbiosis on growth and on the pools of soluble carbohydrates in tobacco plants. New Phytol. 142, 67-77.
- Vijn, I., van Dijken, A., Lüscher, M., Bos, A., Smeets, E., Weisbeek, P., Wiemken, A. and Smeekens, S. (1998) Cloning of Sucrose:Sucrose 1-fructosyltransferase from onion and synthesis of structurally defined fructan molecules from sucrose. Plant Physiol. 117:1507-13
- Wiemken, A., Sprenger, N. and Boller, T. (1995) Fructan - an extension of sucrose by sucrose. In: Pontis H. G., Salerno G. L. and Echeverria E. J., eds., International Symposium on Sucrose Metabolism, pp 179-188
